Hotspot

trnG-UCC-trnR-UCU

LSC · 9274-9406 · 133 bp

Back to Hydrostachys

Pi 0.0758

MarkerSeek score 84.90

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 133
variable_sites: 14
pi: 0.0758
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTATGTCCT	50.0	40.9	58.0	55.6	219-247 bp	1.0	52.9
trnG-UCC-trnR-UCU_p2	AGCCTTCCAAGCTAACGATG	AGAAGACCTATGTCCTATCCA	50.0	42.9	58.0	54.6	213-241 bp	1.0	52.8
trnG-UCC-trnR-UCU_p3	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTATGTCCT	55.0	40.9	58.1	55.6	222-250 bp	1.0	52.5
trnG-UCC-trnR-UCU_p4	CCTAGCCTTCCAAGCTAACG	AGAAGACCTATGTCCTATCCA	55.0	42.9	58.1	54.6	216-244 bp	1.0	52.5
trnG-UCC-trnR-UCU_p5	AGCCTTCCAAGCTAACGATG	ACCAAAGGTTTAGAAGACCTATGT	50.0	37.5	58.0	57.6	224-252 bp	1.0	52.1