Hotspot

psbI-trnS-GCU

LSC · 7576-7733 · 158 bp

Back to Hydrostachys

Pi 0.0743

MarkerSeek score 78.80

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 158
variable_sites: 16
pi: 0.0743
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	GCAATAAAAATGGCGCACCT	TGGACTAAAGCGTCGGATTG	45.0	50.0	58.0	58.0	494-505 bp	1.0	86.4
psbI-trnS-GCU_p2	GCAATAAAAATGGCGCACCT	GTGGACTAAAGCGTCGGATT	45.0	50.0	58.0	58.0	495-506 bp	1.0	86.4
psbI-trnS-GCU_p3	GTTTGGCAAGCTGCTGTAAG	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.9	58.0	655-666 bp	1.0	86.3
psbI-trnS-GCU_p4	GTTTGGCAAGCTGCTGTAAG	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.9	58.0	656-667 bp	1.0	86.3
psbI-trnS-GCU_p5	GCAATAAAAATGGCGCACCT	TAGGAGAGATGGCTGAGTGG	45.0	55.0	58.0	57.9	511-522 bp	1.0	85.6