Hotspot

rps16-trnQ-UUG

LSC · 6267-6546 · 280 bp

Back to Hydrostachys

Pi 0.086

MarkerSeek score 78.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 280
variable_sites: 36
pi: 0.086
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GTCTTTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	360-404 bp	1.0	87.1
rps16-trnQ-UUG_p2	ACCGGATCGTGTCTTTCAAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	370-414 bp	1.0	86.3
rps16-trnQ-UUG_p3	CCGGATCGTGTCTTTCAAGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	369-413 bp	1.0	86.3
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	353-397 bp	1.0	86.2
rps16-trnQ-UUG_p5	ATCGTGTCTTTCAAGTCGCA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.9	58.1	365-409 bp	1.0	85.8