Hotspot

trnK-UUU-rps16

LSC · 4048-5133 · 1086 bp

Back to Hydrostachys

Pi 0.0738
MarkerSeek score 86.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1086
variable_sites
110
pi
0.0738
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
86.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCGCACTTAAAAGCCGAG TCAAAGAGCATCAATTTAATCA 50.0 27.3 58.6 52.1 1146-1169 bp 1.0 51.6
trnK-UUU-rps16_p2 GAGTCGCACTTAAAAGCCGA TCAAAGAGCATCAATTTAATCA 50.0 27.3 58.6 52.1 1147-1170 bp 1.0 51.6
trnK-UUU-rps16_p3 TCGAGTCGCACTTAAAAGCC TCAAAGAGCATCAATTTAATCA 50.0 27.3 58.6 52.1 1149-1172 bp 1.0 51.6
trnK-UUU-rps16_p4 AGTCGCACTTAAAAGCCGAG TCAAAGAGCATCAATTTAATCAA 50.0 26.1 58.6 53.0 1146-1169 bp 1.0 51.6
trnK-UUU-rps16_p5 AGTCGCACTTAAAAGCCGAG TTCAAAGAGCATCAATTTAATCA 50.0 26.1 58.6 53.0 1147-1170 bp 1.0 51.6