Hotspot

ycf4

Genome · 137296-137841 · 546 bp

Back to Hydrolithon

Pi 0.022

MarkerSeek score 56.10

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: Genome
length_bp: 546
variable_sites: 12
pi: 0.022
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	AACCCGCATTACTAGCTTGG	CGGTAAGCAGGCAAGTTACT	50.0	50.0	58.0	57.9	1189 bp	1.0	81.0
ycf4_p2	GGAATCGAACCCGCATTACT	CGGTAAGCAGGCAAGTTACT	50.0	50.0	58.1	57.9	1196 bp	1.0	80.9
ycf4_p3	CGAACCCGCATTACTAGCTT	CGGTAAGCAGGCAAGTTACT	50.0	50.0	58.1	57.9	1191 bp	1.0	80.8
ycf4_p4	AACCCGCATTACTAGCTTGG	AGTATCCATGGGCCATACCA	50.0	50.0	58.0	57.9	1245 bp	1.0	80.8
ycf4_p5	GGAATCGAACCCGCATTACT	AGTATCCATGGGCCATACCA	50.0	50.0	58.1	57.9	1252 bp	1.0	80.6