Hotspot

ntcA

Genome · 41389-41946 · 558 bp

Back to Hydrolithon

Pi 0.0287

MarkerSeek score 59.40

Primer available yes

Label ntcA

Feature Metadata

label_name: ntcA
feature_type: gene
region: Genome
length_bp: 558
variable_sites: 16
pi: 0.0287
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ntcA_p1	CAAGAGGTTACGGCAATGGA	GGGTTCCCACAACTGGAATT	50.0	50.0	57.9	58.0	1893 bp	1.0	80.6
ntcA_p2	ATTATTCGCGGGTTCGAGTC	GGGTTCCCACAACTGGAATT	50.0	50.0	58.2	58.0	1863 bp	1.0	80.3
ntcA_p3	CAAGAGGTTACGGCAATGGA	GCTGCCCAATAGCCAATTTG	50.0	50.0	57.9	58.1	1846 bp	1.0	80.2
ntcA_p4	CAAGAGGTTACGGCAATGGA	TTCCCACAACTGGAATTGCA	50.0	45.0	57.9	57.9	1890 bp	1.0	80.0
ntcA_p5	ATTATTCGCGGGTTCGAGTC	GCTGCCCAATAGCCAATTTG	50.0	50.0	58.2	58.1	1816 bp	1.0	79.9