Hotspot

infB

Genome · 110079-112196 · 2118 bp

Back to Hydrolithon

Pi 0.0151

MarkerSeek score 51.40

Primer available yes

Label infB

Feature Metadata

label_name: infB
feature_type: gene
region: Genome
length_bp: 2118
variable_sites: 32
pi: 0.0151
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infB_p1	TCTGCAAGCACAGTGAAAGT	TGTCCTAATTGTAATCATCACCGT	45.0	37.5	58.0	58.0	2691-2692 bp	1.0	48.9
infB_p2	TTCACCCTCAGATTGTGCTG	TGTCCTAATTGTAATCATCACCGT	50.0	37.5	57.8	58.0	2782-2783 bp	1.0	47.7
infB_p3	CGCGGCATAAGCTTTCTTTT	TGTCCTAATTGTAATCATCACCGT	45.0	37.5	57.7	58.0	2640-2641 bp	1.0	47.1
infB_p4	TCTGCAAGCACAGTGAAAGT	TGACGAAAAATAAAGGTTCTCGT	45.0	34.8	58.0	56.8	2843-2844 bp	1.0	47.0
infB_p5	ATCGCGGCATAAGCTTTCTT	TGTCCTAATTGTAATCATCACCGT	45.0	37.5	58.3	58.0	2642-2643 bp	1.0	46.6