Hotspot

ycf22-psaC

Genome · 154062-154595 · 534 bp

Back to Hydrolithon

Pi 0.0356
MarkerSeek score 65.50
Primer available yes
Label ycf22-psaC

Feature Metadata

label_name
ycf22-psaC
feature_type
igs
region
Genome
length_bp
534
variable_sites
19
pi
0.0356
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf22-psaC_p1 ACTTTGTATATTTATATGGGAGCT CAACAAGAAGTATGGGTCTAGCT 29.2 43.5 53.3 57.9 608-610 bp 1.0 42.5
ycf22-psaC_p2 ACTTTGTATATTTATATGGGAGCT ACAACAAGAAGTATGGGTCTAGC 29.2 43.5 53.3 58.2 609-611 bp 1.0 42.5
ycf22-psaC_p3 ACTTTGTATATTTATATGGGAGCT GCTGAAACAACAAGAAGTATGGG 29.2 43.5 53.3 58.2 615-617 bp 1.0 42.5
ycf22-psaC_p4 ACTTTGTATATTTATATGGGAGCT ACAAGAAGTATGGGTCTAGCT 29.2 42.9 53.3 55.8 606-608 bp 1.0 42.5
ycf22-psaC_p5 AACTTTGTATATTTATATGGGAGCT CAACAAGAAGTATGGGTCTAGCT 28.0 43.5 54.1 57.9 609-611 bp 1.0 42.5