Hotspot

apcB-atpE

Genome · 107488-107539 · 52 bp

Back to Hydrolithon

Pi 0.0962

MarkerSeek score 85.10

Primer available yes

Label apcB-atpE

Feature Metadata

label_name: apcB-atpE
feature_type: igs
region: Genome
length_bp: 52
variable_sites: 5
pi: 0.0962
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 85.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
apcB-atpE_p1	TGGTAGGCTTAGATGCTGGA	AGAAAAGCAAGAGCACGTGT	50.0	45.0	57.8	58.3	168 bp	1.0	78.3
apcB-atpE_p2	TGGTAGGCTTAGATGCTGGA	AAAAGCAAGAGCACGTGTTC	50.0	45.0	57.8	57.5	166 bp	1.0	77.2
apcB-atpE_p3	TGGTAGGCTTAGATGCTGGA	GAAAAGCAAGAGCACGTGTT	50.0	45.0	57.8	57.5	167 bp	1.0	77.2
apcB-atpE_p4	ACAACTGCTTTGGTAGGCTT	AGAAAAGCAAGAGCACGTGT	45.0	45.0	57.6	58.3	178 bp	1.0	76.7
apcB-atpE_p5	TGGTAGGCTTAGATGCTGGA	AAGCAAGAGCACGTGTTCAA	50.0	45.0	57.8	58.6	164 bp	1.0	76.1