Hotspot

tatC-apcE

Genome · 102422-103663 · 1242 bp

Back to Hydrolithon

Pi 0.0306

MarkerSeek score 62.00

Primer available yes

Label tatC-apcE

Feature Metadata

label_name: tatC-apcE
feature_type: igs
region: Genome
length_bp: 1242
variable_sites: 38
pi: 0.0306
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tatC-apcE_p1	TTGATGGCGTTAGGATTGCA	ATCGTTGATGCTGATGCTGT	45.0	45.0	57.9	58.0	1980-1995 bp	1.0	80.8
tatC-apcE_p2	ATGGCGTTAGGATTGCACTT	ATCGTTGATGCTGATGCTGT	45.0	45.0	57.9	58.0	1977-1992 bp	1.0	80.8
tatC-apcE_p3	TTGATGGCGTTAGGATTGCA	CTATTGCTGCCGCACTAGAA	45.0	50.0	57.9	58.1	2483-2498 bp	1.0	80.5
tatC-apcE_p4	ATGGCGTTAGGATTGCACTT	CTATTGCTGCCGCACTAGAA	45.0	50.0	57.9	58.1	2480-2495 bp	1.0	80.4
tatC-apcE_p5	TTGATGGCGTTAGGATTGCA	TGAGCAATCGTTGATGCTGA	45.0	45.0	57.9	57.9	1986-2001 bp	1.0	80.3