Hotspot

psaD-chlB

Genome · 88976-89153 · 178 bp

Back to Hydrolithon

Pi 0.0506

MarkerSeek score 76.80

Primer available yes

Label psaD-chlB

Feature Metadata

label_name: psaD-chlB
feature_type: igs
region: Genome
length_bp: 178
variable_sites: 9
pi: 0.0506
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaD-chlB_p1	AATGTTGGTGCTGGCATAGT	GAGCTGGTCCTGCATACATC	45.0	55.0	57.8	58.1	283 bp	1.0	79.8
psaD-chlB_p2	AATGTTGGTGCTGGCATAGT	CAATATGAGCTGGTCCTGCA	45.0	50.0	57.8	57.7	289 bp	1.0	78.0
psaD-chlB_p3	GCAGATCTTAGCCATCCTCC	GAGCTGGTCCTGCATACATC	55.0	55.0	57.5	58.1	316 bp	1.0	77.5
psaD-chlB_p4	AATGTTGGTGCTGGCATAGT	CCAATATGAGCTGGTCCTGC	45.0	55.0	57.8	58.4	290 bp	1.0	77.5
psaD-chlB_p5	CCAGTACTGCCTCCAAATGT	GAGCTGGTCCTGCATACATC	50.0	55.0	57.5	58.1	298 bp	1.0	77.5