Hotspot

rpl13-rpoA

Genome · 66392-66451 · 60 bp

Back to Hydrolithon

Pi 0.05

MarkerSeek score 75.20

Primer available yes

Label rpl13-rpoA

Feature Metadata

label_name: rpl13-rpoA
feature_type: igs
region: Genome
length_bp: 60
variable_sites: 3
pi: 0.05
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl13-rpoA_p1	TGTGCTTAGTCTGCCTAATGT	CTGCTGAAGAAGTTATTGAAGCC	42.9	43.5	57.0	58.0	224 bp	1.0	41.1
rpl13-rpoA_p2	TGTGCTTAGTCTGCCTAATGT	TGCTGAAGAAGTTATTGAAGCC	42.9	40.9	57.0	57.0	223 bp	1.0	41.1
rpl13-rpoA_p3	GCGATTAATGTGCTTAGTCTGC	CTGCTGAAGAAGTTATTGAAGCC	45.5	43.5	58.1	58.0	232 bp	1.0	41.0
rpl13-rpoA_p4	GCGATTAATGTGCTTAGTCTGC	TGCTGAAGAAGTTATTGAAGCC	45.5	40.9	58.1	57.0	231 bp	1.0	41.0
rpl13-rpoA_p5	TGTGCTTAGTCTGCCTAATGT	ACTTTGGACAAAAATCTGCTGA	42.9	36.4	57.0	56.7	239 bp	1.0	41.0