Hotspot

trnS-GCU-trnD-GUC

Genome · 56136-56607 · 472 bp

Back to Hydrolithon

Pi 0.221

MarkerSeek score 88.80

Primer available yes

Label trnS-GCU-trnD-GUC

Feature Metadata

label_name: trnS-GCU-trnD-GUC
feature_type: igs
region: Genome
length_bp: 472
variable_sites: 99
pi: 0.221
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 88.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnD-GUC_p1	GAGAGATGGCTGAGTGGTTG	GGGCGATGCTCTAACCAATT	55.0	50.0	58.0	58.3	587-595 bp	1.0	88.7
trnS-GCU-trnD-GUC_p2	GGTTGAAAGCGTCAGATTGC	GGGCGATGCTCTAACCAATT	50.0	50.0	58.0	58.3	572-580 bp	1.0	88.7
trnS-GCU-trnD-GUC_p3	TAGGAGAGATGGCTGAGTGG	GGGCGATGCTCTAACCAATT	55.0	50.0	57.9	58.3	590-598 bp	1.0	88.2
trnS-GCU-trnD-GUC_p4	GAGAGATGGCTGAGTGGTTG	TCGCATTCGGTAGCAGATAC	55.0	50.0	58.0	57.6	658-666 bp	1.0	87.5
trnS-GCU-trnD-GUC_p5	GAGAGATGGCTGAGTGGTTG	CGCATTCGGTAGCAGATACT	55.0	50.0	58.0	57.6	657-665 bp	1.0	87.5