Hotspot

rps16-groEL

Genome · 32658-33520 · 863 bp

Back to Hydrolithon

Pi 0.022

MarkerSeek score 60.90

Primer available yes

Label rps16-groEL

Feature Metadata

label_name: rps16-groEL
feature_type: igs
region: Genome
length_bp: 863
variable_sites: 19
pi: 0.022
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-groEL_p1	ACAAACACTGTTCACTATCT	TGCAGGTATTGTTGATCCGG	35.0	50.0	52.0	58.0	1029-1034 bp	1.0	41.5
rps16-groEL_p2	ACAAACACTGTTCACTATCTT	TGCAGGTATTGTTGATCCGG	33.3	50.0	53.0	58.0	1029-1034 bp	1.0	41.5
rps16-groEL_p3	ACAAACACTGTTCACTATCT	ACTCGTTCAGCATTGCAGAA	35.0	45.0	52.0	58.1	1001-1006 bp	1.0	41.5
rps16-groEL_p4	ACAAACACTGTTCACTATCTT	ACTCGTTCAGCATTGCAGAA	33.3	45.0	53.0	58.1	1001-1006 bp	1.0	41.5
rps16-groEL_p5	ACAAACACTGTTCACTATCT	CAGGTATTGTTGATCCGGCT	35.0	50.0	52.0	57.7	1027-1032 bp	1.0	41.5