Hotspot

psbA-rne

Genome · 5908-5994 · 87 bp

Back to Hydrolithon

Pi 0.128

MarkerSeek score 81.50

Primer available yes

Label psbA-rne

Feature Metadata

label_name: psbA-rne
feature_type: igs
region: Genome
length_bp: 87
variable_sites: 11
pi: 0.128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-rne_p1	AATGCACACAACTTCCCACT	CCCATTCATTGTACCGCTGA	45.0	50.0	57.9	58.0	231-232 bp	1.0	83.3
psbA-rne_p2	TGCACACAACTTCCCACTAG	CCCATTCATTGTACCGCTGA	50.0	50.0	57.7	58.0	229-230 bp	1.0	81.9
psbA-rne_p3	AATGCACACAACTTCCCACT	ACCCATTCATTGTACCGCTG	45.0	50.0	57.9	58.2	232-233 bp	1.0	81.6
psbA-rne_p4	AATGCACACAACTTCCCACT	CACCCATTCATTGTACCGCT	45.0	50.0	57.9	58.2	233-234 bp	1.0	81.5
psbA-rne_p5	AATGCACACAACTTCCCACT	TCACCCATTCATTGTACCGC	45.0	50.0	57.9	58.3	234-235 bp	1.0	81.5