Hotspot

ccs1-trnN-GUU

Genome · 3897-4419 · 523 bp

Back to Hydrolithon

Pi 0.0363

MarkerSeek score 67.50

Primer available yes

Label ccs1-trnN-GUU

Feature Metadata

label_name: ccs1-trnN-GUU
feature_type: igs
region: Genome
length_bp: 523
variable_sites: 19
pi: 0.0363
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccs1-trnN-GUU_p1	GCAAACCGTAAGCTACTAATCC	GGTTCAAATCCTTCCTGGGG	45.5	55.0	57.2	58.1	604-611 bp	1.0	59.0
ccs1-trnN-GUU_p2	GCAAACCGTAAGCTACTAATCC	CTCAGTGGTAGAGCAATCGG	45.5	55.0	57.2	57.8	644-651 bp	1.0	58.3
ccs1-trnN-GUU_p3	GCAAACCGTAAGCTACTAATCC	TTCCTCAGTAGCTCAGTGGT	45.5	50.0	57.2	57.7	655-662 bp	1.0	57.5
ccs1-trnN-GUU_p4	GCAAACCGTAAGCTACTAATCC	CCGATTGGTCGTAGGTTCAA	45.5	50.0	57.2	57.6	617-624 bp	1.0	57.1
ccs1-trnN-GUU_p5	ACCGTAAGCTACTAATCCACT	GGTTCAAATCCTTCCTGGGG	42.9	55.0	55.9	58.1	600-607 bp	1.0	56.5