Hotspot

ndhF-rpl32

SSC · 111659-112875 · 1217 bp

Back to Hydrocotyle

Pi 0.0196

MarkerSeek score 63.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1217
variable_sites: 106
pi: 0.0196
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGGGTCGATCAAGTATCCGA	TTTAAGGCCGCCCAATATCC	50.0	50.0	57.9	58.0	1503-1631 bp	1.0	89.0
ndhF-rpl32_p2	TGGGTCGATCAAGTATCCGA	CCCACCCCCAAAATGAGAAA	50.0	50.0	57.9	58.0	1733-1863 bp	1.0	88.8
ndhF-rpl32_p3	AACCAAAATCCCCAACACGA	TTTAAGGCCGCCCAATATCC	45.0	50.0	57.9	58.0	1795-1923 bp	1.0	88.3
ndhF-rpl32_p4	TCCCCAACACGATTCGTTAC	TTTAAGGCCGCCCAATATCC	50.0	50.0	57.9	58.0	1787-1915 bp	1.0	88.2
ndhF-rpl32_p5	AACCAAAATCCCCAACACGA	CCCACCCCCAAAATGAGAAA	45.0	50.0	57.9	58.0	2025-2155 bp	1.0	88.1