Hotspot

rpl22-rps19

LSC · 83900-84077 · 178 bp

Back to Hydrocotyle

Pi 0.0653

MarkerSeek score 72.00

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 178
variable_sites: 42
pi: 0.0653
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CGTAGGAACGTCCACGAATT	TCCCGGGCATCTACCATTAT	50.0	50.0	58.0	58.0	456-507 bp	1.0	91.6
rpl22-rps19_p2	CGTAGGAACGTCCACGAATT	GGTTATCCTGCACTTGGGAG	50.0	55.0	58.0	58.0	703-752 bp	1.0	89.6
rpl22-rps19_p3	CGTAGGAACGTCCACGAATT	TGGGTAGAGCCGGATCTAAG	50.0	55.0	58.0	58.0	840-889 bp	1.0	89.0
rpl22-rps19_p4	TGCTGCTGCAGAATAAACCA	TGGGTAGAGCCGGATCTAAG	45.0	55.0	58.1	58.0	920-969 bp	1.0	88.1
rpl22-rps19_p5	GCAATTCCCTCAACACTGGA	TGGGTAGAGCCGGATCTAAG	50.0	55.0	58.1	58.0	1380-1432 bp	1.0	87.3