Hotspot

psbT-psbN

LSC · 74271-74353 · 83 bp

Back to Hydrocotyle

Pi 0.042

MarkerSeek score 58.30

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 83
variable_sites: 11
pi: 0.042
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TGGCGACGGAATTGTTAGAG	AAAATTCCGCGGTCGAACTA	50.0	45.0	58.0	57.9	1267-1280 bp	1.0	83.7
psbT-psbN_p2	TGGCGACGGAATTGTTAGAG	CTGGTTGGTTTTGGACGAGA	50.0	50.0	58.0	58.0	997-1009 bp	1.0	83.5
psbT-psbN_p3	TGTACGTCGTATGCCTACCT	CTGGTTGGTTTTGGACGAGA	50.0	50.0	58.0	58.0	1048-1060 bp	1.0	83.1
psbT-psbN_p4	GGAATAGCGGTTGGATGGTT	CTGGTTGGTTTTGGACGAGA	50.0	50.0	57.9	58.0	1110-1122 bp	1.0	82.9
psbT-psbN_p5	GGGCGTGAACTTTTTGTACG	CTGGTTGGTTTTGGACGAGA	50.0	50.0	58.0	58.0	1062-1074 bp	0.888889	78.4