Hotspot

petA-psbJ

LSC · 63483-64540 · 1058 bp

Back to Hydrocotyle

Pi 0.0217

MarkerSeek score 62.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1058
variable_sites: 87
pi: 0.0217
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CAGGGCCAGAACTTCTTGTT	AACTGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	1825-1878 bp	1.0	87.0
petA-psbJ_p2	CAGGGCCAGAACTTCTTGTT	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1824-1877 bp	1.0	87.0
petA-psbJ_p3	TAGCTCCTGTCGATCGGATT	AACTGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	2203-2256 bp	1.0	86.0
petA-psbJ_p4	TAGCTCCTGTCGATCGGATT	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	2202-2255 bp	1.0	86.0
petA-psbJ_p5	TTAGCTCCTGTCGATCGGAT	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	2203-2256 bp	1.0	86.0