Hotspot

psbM-trnD-GUC

LSC · 30019-31197 · 1179 bp

Back to Hydrocotyle

Pi 0.0115

MarkerSeek score 57.30

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1179
variable_sites: 57
pi: 0.0115
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGCAGAAAAGCAGTAGGAACT	TTCAATTGGTCAGAGCACCG	42.9	50.0	57.2	58.5	1288-1318 bp	1.0	67.3
psbM-trnD-GUC_p2	AAGCAGAAAAGCAGTAGGAACT	TTCAATTGGTCAGAGCACCG	40.9	50.0	57.9	58.5	1289-1319 bp	1.0	64.8
psbM-trnD-GUC_p3	AGCAGAAAAGCAGTAGGAACT	GTTCAATTGGTCAGAGCACC	42.9	50.0	57.2	57.0	1289-1319 bp	1.0	63.1
psbM-trnD-GUC_p4	AGCAGAAAAGCAGTAGGAAC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.7	58.5	1288-1318 bp	1.0	62.9
psbM-trnD-GUC_p5	GCAGAAAAGCAGTAGGAACT	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.7	58.5	1287-1317 bp	1.0	62.9