Hotspot

trnK-UUU-rps16

LSC · 4187-5035 · 849 bp

Back to Hydrocotyle

Pi 0.0164

MarkerSeek score 58.50

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 849
variable_sites: 57
pi: 0.0164
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	TCAGCCTACAGCAACTGTTC	50.0	50.0	58.2	57.8	909-955 bp	1.0	86.2
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	TCAGCCTACAGCAACTGTTC	50.0	50.0	58.2	57.8	908-954 bp	1.0	86.2
trnK-UUU-rps16_p3	AAAGCCGAGTACTCTACCGT	CTCAGCCTACAGCAACTGTT	50.0	50.0	58.2	57.8	910-956 bp	1.0	86.1
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	CTCAGCCTACAGCAACTGTT	50.0	50.0	58.2	57.8	909-955 bp	1.0	86.1
trnK-UUU-rps16_p5	CCGCACTTAAAAGCCGAGTA	TCAGCCTACAGCAACTGTTC	50.0	50.0	58.3	57.8	918-964 bp	1.0	85.2