Hotspot

ndhH

IRb · 114734-115915 · 1182 bp

Back to Hydrocharis

Pi 0.36
MarkerSeek score 61.40
Primer available yes
Label ndhH

Feature Metadata

label_name
ndhH
feature_type
gene
region
IRb
length_bp
1182
variable_sites
41
pi
0.36
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.10
markerseek_score
61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH_p1 TTGATGAACCTCGTTTTGAG AGCTTGTACTTCCATTATCAT 40.0 33.3 53.9 52.3 1257 bp 0.333333 13.3
ndhH_p2 TTGATGAACCTCGTTTTGAG AGCTTGTACTTCCATTATCATT 40.0 31.8 53.9 53.2 1257 bp 0.333333 13.3
ndhH_p3 TTTGATGAACCTCGTTTTGAG AGCTTGTACTTCCATTATCAT 38.1 33.3 54.7 52.3 1258 bp 0.333333 13.3
ndhH_p4 TTTGATGAACCTCGTTTTGAG AGCTTGTACTTCCATTATCATT 38.1 31.8 54.7 53.2 1258 bp 0.333333 13.3
ndhH_p5 TTTTGATGAACCTCGTTTTGAG AGCTTGTACTTCCATTATCAT 36.4 33.3 55.5 52.3 1259 bp 0.333333 13.3