Hotspot

ndhF-rpl32

SSC · 118372-119359 · 988 bp

Back to Hydrocharis

Pi 0.0846

MarkerSeek score 87.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 988
variable_sites: 117
pi: 0.0846
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 87.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACAGGAAGTGGAAGGAAAGG	AGCCTTAACTGCAGCCAAAT	50.0	45.0	57.0	58.1	1134-1244 bp	1.0	83.7
ndhF-rpl32_p2	CAGGAAGTGGAAGGAAAGGT	AGCCTTAACTGCAGCCAAAT	50.0	45.0	57.0	58.1	1133-1243 bp	1.0	83.7
ndhF-rpl32_p3	CAGGAAGTGGAAGGAAAGGT	AAAAGCCTTAACTGCAGCCA	50.0	45.0	57.0	58.3	1136-1246 bp	1.0	81.9
ndhF-rpl32_p4	CAGGAAGTGGAAGGAAAGGT	AAAGCCTTAACTGCAGCCAA	50.0	45.0	57.0	58.3	1135-1245 bp	1.0	81.9
ndhF-rpl32_p5	CAGGAAGTGGAAGGAAAGGT	AAGCCTTAACTGCAGCCAAA	50.0	45.0	57.0	58.3	1134-1244 bp	1.0	81.9