Hotspot

psaA-ycf3

LSC · 42145-42731 · 587 bp

Back to Hydrocharis

Pi 0.0757

MarkerSeek score 83.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 587
variable_sites: 63
pi: 0.0757
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TTTCACTTCTTGTTCCGGCG	TTGGTTGAAGATCACAGGGC	50.0	50.0	59.1	58.1	651-675 bp	1.0	80.5
psaA-ycf3_p2	TTTTCACTTCTTGTTCCGGC	TTGGTTGAAGATCACAGGGC	45.0	50.0	56.9	58.1	652-676 bp	1.0	79.7
psaA-ycf3_p3	CACTTCTTGTTCCGGCGAA	TTGGTTGAAGATCACAGGGC	52.6	50.0	58.4	58.1	648-672 bp	1.0	77.9
psaA-ycf3_p4	TTCACTTCTTGTTCCGGCG	TTGGTTGAAGATCACAGGGC	52.6	50.0	58.4	58.1	650-674 bp	1.0	77.8
psaA-ycf3_p5	ATTTTCACTTCTTGTTCCGGC	TTGGTTGAAGATCACAGGGC	42.9	50.0	57.4	58.1	653-677 bp	1.0	75.8