Hotspot

trnD-GUC-trnY-GUA

LSC · 30911-31254 · 344 bp

Back to Hydrocharis

Pi 0.0645
MarkerSeek score 82.90
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
344
variable_sites
32
pi
0.0645
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 CGGTGCTCTGACCAATTGAA GGGGACGGACTGTAAATTCG 50.0 55.0 58.5 58.4 425-443 bp 1.0 83.3
trnD-GUC-trnY-GUA_p2 CGGTGCTCTGACCAATTGAA GGGACGGACTGTAAATTCGT 50.0 50.0 58.5 57.6 424-442 bp 1.0 83.1
trnD-GUC-trnY-GUA_p3 CGGTGCTCTGACCAATTGAA GATTCTTCCTGGGTCGATGC 50.0 55.0 58.5 58.4 458-476 bp 1.0 82.4
trnD-GUC-trnY-GUA_p4 CGGTGCTCTGACCAATTGAA TGGGGACGGACTGTAAATTC 50.0 50.0 58.5 57.2 426-444 bp 1.0 79.9
trnD-GUC-trnY-GUA_p5 GGTGCTCTGACCAATTGAAC GGGGACGGACTGTAAATTCG 50.0 55.0 57.0 58.4 424-442 bp 1.0 79.1