Hotspot

rps2-rpoC2

LSC · 15934-16563 · 630 bp

Back to Hydrocharis

Pi 0.17

MarkerSeek score 83.70

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 630
variable_sites: 151
pi: 0.17
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	CATCATTGGCAGGAATCGGA	TTATCGATCCCAGGGGGTAC	50.0	55.0	58.0	58.0	1742 bp	0.333333	52.9
rps2-rpoC2_p2	TCATCATTGGCAGGAATCGG	TTATCGATCCCAGGGGGTAC	50.0	55.0	58.0	58.0	1743 bp	0.333333	52.9
rps2-rpoC2_p3	CTCCTGCTTTGATCATGCCT	TTATCGATCCCAGGGGGTAC	50.0	55.0	57.9	58.0	1169 bp	0.333333	52.7
rps2-rpoC2_p4	TGACCAAAATGGACTCCTGC	TTATCGATCCCAGGGGGTAC	50.0	55.0	58.1	58.0	1182 bp	0.333333	52.5
rps2-rpoC2_p5	ACCGTGACCAAAATGGACTC	TTATCGATCCCAGGGGGTAC	50.0	55.0	58.1	58.0	1186 bp	0.333333	52.3