Hotspot

trnS-GCU-trnG-UCC

LSC · 8046-8811 · 766 bp

Back to Hydrocharis

Pi 0.0865

MarkerSeek score 85.00

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 766
variable_sites: 90
pi: 0.0865
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 85.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	AGTCCACTCAGCCATCTCTC	ACGAATCACACTTTTACCACT	55.0	38.1	58.5	55.5	821-952 bp	1.0	59.5
trnS-GCU-trnG-UCC_p2	AGTCCACTCAGCCATCTCTC	GAACGAATCACACTTTTACCACT	55.0	39.1	58.5	57.3	823-954 bp	1.0	58.5
trnS-GCU-trnG-UCC_p3	AGTCCACTCAGCCATCTCTC	AGAACGAATCACACTTTTACCAC	55.0	39.1	58.5	57.3	824-955 bp	1.0	58.4
trnS-GCU-trnG-UCC_p4	AGTCCACTCAGCCATCTCTC	AACGAATCACACTTTTACCACT	55.0	36.4	58.5	56.2	822-953 bp	1.0	57.6
trnS-GCU-trnG-UCC_p5	TAGTCCACTCAGCCATCTCT	ACGAATCACACTTTTACCACT	50.0	38.1	57.2	55.5	822-953 bp	1.0	57.0