Hotspot

trnK-UUU-trnQ-UUG

LSC · 4204-6415 · 2212 bp

Back to Hydrocharis

Pi 0.277
MarkerSeek score 80.40
Primer available yes
Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name
trnK-UUU-trnQ-UUG
feature_type
igs
region
LSC
length_bp
2212
variable_sites
778
pi
0.277
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.94
markerseek_score
80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-trnQ-UUG_p1 CAGGATCAGTCGTGGTCTTG GACAAGTCGGCCTAGATGTG 55.0 55.0 58.0 58.1 2457 bp 0.333333 52.7
trnK-UUU-trnQ-UUG_p2 CAGGATCAGTCGTGGTCTTG TCTTGACAAGTCGGCCTAGA 55.0 50.0 58.0 58.1 2461 bp 0.333333 52.7
trnK-UUU-trnQ-UUG_p3 CCTTTCAGGATCAGTCGTGG GACAAGTCGGCCTAGATGTG 55.0 55.0 58.0 58.1 2462 bp 0.333333 52.6
trnK-UUU-trnQ-UUG_p4 CCTTTCAGGATCAGTCGTGG TCTTGACAAGTCGGCCTAGA 55.0 50.0 58.0 58.1 2466 bp 0.333333 52.6
trnK-UUU-trnQ-UUG_p5 CAGGATCAGTCGTGGTCTTG TCAACGCCTCTTGACAAGTC 55.0 50.0 58.0 58.1 2469 bp 0.333333 52.2