Hotspot

ycf1

SSC · 127463-132895 · 5433 bp

Back to Hydriastele

Pi 0.00497

MarkerSeek score 55.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5433
variable_sites: 27
pi: 0.00497
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCCAAGTTTCTGATGTTGCT	TCCATGCGTACTCAAAGACG	42.9	50.0	57.8	58.0	269 bp	1.0	71.9
ycf1_p2	TCCCAAGTTTCTGATGTTGCT	CGCAACAATCGGATTTTCGT	42.9	45.0	57.8	57.8	307 bp	1.0	69.8
ycf1_p3	TCCCAAGTTTCTGATGTTGCT	CCATGCGTACTCAAAGACGT	42.9	50.0	57.8	58.3	268 bp	1.0	69.6
ycf1_p4	TCCCAAGTTTCTGATGTTGCT	GCTACTCGCAACAATCGGAT	42.9	50.0	57.8	58.4	313 bp	1.0	68.3
ycf1_p5	TCCCAAGTTTCTGATGTTGCT	TCGCAACAATCGGATTTTCG	42.9	45.0	57.8	57.5	308 bp	1.0	67.6