Hotspot

psbA

LSC · 246-1307 · 1062 bp

Back to Hydriastele

Pi 0.000942

MarkerSeek score 54.60

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 1
pi: 0.000942
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TCAAACAATGGAGCAATACCCA	GTGCTTGGGAGTCCTTGAAA	40.9	50.0	58.2	58.0	1196 bp	1.0	62.1
psbA_p2	CAAACAATGGAGCAATACCCA	GTGCTTGGGAGTCCTTGAAA	42.9	50.0	56.8	58.0	1195 bp	1.0	62.0
psbA_p3	TCAAACAATGGAGCAATACCCA	AATACGTGTGCTTGGGAGTC	40.9	50.0	58.2	57.9	1203 bp	1.0	61.5
psbA_p4	CAAACAATGGAGCAATACCCA	AATACGTGTGCTTGGGAGTC	42.9	50.0	56.8	57.9	1202 bp	1.0	61.4
psbA_p5	TCAAACAATGGAGCAATACCC	GTGCTTGGGAGTCCTTGAAA	42.9	50.0	56.5	58.0	1196 bp	1.0	59.8