Hotspot

accD

LSC · 59728-61260 · 1533 bp

Back to Hydriastele

Pi 0.00459

MarkerSeek score 49.60

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1533
variable_sites: 7
pi: 0.00459
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	ATAGGGGGCAAGAAGACTCT	TGTTCGCTACAAATAACTGA	50.0	35.0	57.4	52.6	1579-1588 bp	1.0	40.6
accD_p2	ATAGGGGGCAAGAAGACTCT	TGTTCGCTACAAATAACTGAA	50.0	33.3	57.4	53.5	1579-1588 bp	1.0	40.6
accD_p3	ATAGGGGGCAAGAAGACTCT	TTGTTCGCTACAAATAACTGA	50.0	33.3	57.4	53.5	1580-1589 bp	1.0	40.6
accD_p4	ATAGGGGGCAAGAAGACTCT	TTGTTCGCTACAAATAACTGAA	50.0	31.8	57.4	54.4	1580-1589 bp	1.0	40.6
accD_p5	ATAGGGGGCAAGAAGACTCT	TGTTCGCTACAAATAACTGAAT	50.0	31.8	57.4	54.1	1579-1588 bp	1.0	40.6