Hotspot

psaC-ndhE

SSC · 120816-121394 · 579 bp

Back to Hydriastele

Pi 0.0123

MarkerSeek score 66.70

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 579
variable_sites: 7
pi: 0.0123
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	ACATCCGTCCCAAGGTATCA	TGTTATAGCTGTTGCGGCTG	50.0	50.0	58.1	58.6	793-800 bp	1.0	75.4
psaC-ndhE_p2	CAATACATCTGTGGGGCAGG	TGTTATAGCTGTTGCGGCTG	55.0	50.0	58.3	58.6	769-776 bp	1.0	74.0
psaC-ndhE_p3	CCAATACATCTGTGGGGCAG	TGTTATAGCTGTTGCGGCTG	55.0	50.0	58.3	58.6	770-777 bp	1.0	74.0
psaC-ndhE_p4	CAGGCTCGTACACATTGAGT	TGTTATAGCTGTTGCGGCTG	50.0	50.0	57.6	58.6	753-760 bp	1.0	73.6
psaC-ndhE_p5	AGCTTTACATCCGTCCCAAG	TGTTATAGCTGTTGCGGCTG	50.0	50.0	57.6	58.6	799-806 bp	1.0	73.2