Hotspot

rpl32-trnL-UAG

SSC · 116813-117601 · 789 bp

Back to Hydriastele

Pi 0.0051
MarkerSeek score 62.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
789
variable_sites
4
pi
0.0051
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGTTTCCACCGGACATTCAA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.4 58.0 879-882 bp 1.0 77.6
rpl32-trnL-UAG_p2 GGTTTCCACCGGACATTCAA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.4 58.0 880-883 bp 1.0 77.6
rpl32-trnL-UAG_p3 GGTTTCCACCGGACATTCAA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.4 57.9 889-892 bp 1.0 76.7
rpl32-trnL-UAG_p4 GGTTTCCACCGGACATTCAA CCTAAGAGCAGCGTGTCTAC 50.0 55.0 58.4 57.8 888-891 bp 1.0 76.1
rpl32-trnL-UAG_p5 GGTTTCCACCGGACATTCAA CTAAGAGCAGCGTGTCTACC 50.0 55.0 58.4 57.8 887-890 bp 1.0 76.1