Hotspot

psbE-petL

LSC · 67126-68404 · 1279 bp

Back to Hydriastele

Pi 0.00313

MarkerSeek score 47.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1279
variable_sites: 4
pi: 0.00313
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.9	58.0	1963-1970 bp	1.0	79.8
psbE-petL_p2	ACACGTCGTAAGCTAAACCC	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.9	58.0	1998-2005 bp	1.0	79.7
psbE-petL_p3	ACTGACGAATAACCAACCCG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.9	58.0	1976-1983 bp	1.0	79.5
psbE-petL_p4	GAACAGCCAACCATCGTACT	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.9	58.0	2174-2181 bp	1.0	79.4
psbE-petL_p5	TCAAAACGGCCGGTTATCAA	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.1	58.0	2071-2078 bp	1.0	79.4