Hotspot

trnR-UCU-atpA

LSC · 10759-10849 · 91 bp

Back to Hydriastele

Pi 0.022

MarkerSeek score 65.10

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 91
variable_sites: 2
pi: 0.022
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	ACTTCAGGAACAAGCGTCAA	47.6	45.0	57.3	57.7	153 bp	1.0	64.9
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	TCAGGAACAAGCGTCAACTT	47.6	45.0	57.3	57.7	150 bp	1.0	64.9
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	TTCAGGAACAAGCGTCAACT	47.6	45.0	57.3	57.7	151 bp	1.0	64.9
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	AACAAGCGTCAACTTCTCCC	47.6	50.0	57.3	58.4	145 bp	1.0	64.3
trnR-UCU-atpA_p5	GGTTCAAATCCTATTGGACGC	TACCAAGACATTCACCGAGC	47.6	50.0	57.3	57.6	225 bp	1.0	64.1