Hotspot

trnK-UUU-rps16

LSC · 4218-4937 · 720 bp

Back to Hydriastele

Pi 0.00557

MarkerSeek score 59.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 720
variable_sites: 4
pi: 0.00557
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCTGGACGAATCTGTTGC	GCTCAACCTACGGGAACTG	50.0	57.9	57.9	57.9	889-891 bp	1.0	70.6
trnK-UUU-rps16_p2	GTCTGGACGAATCTGTTGCT	GCTCAACCTACGGGAACTG	50.0	57.9	57.9	57.9	888-890 bp	1.0	70.6
trnK-UUU-rps16_p3	AGTCTGGACGAATCTGTTGC	GTGCTCAACCTACGGGAAC	50.0	57.9	57.9	58.2	891-893 bp	1.0	70.5
trnK-UUU-rps16_p4	GTCTGGACGAATCTGTTGCT	GTGCTCAACCTACGGGAAC	50.0	57.9	57.9	58.2	890-892 bp	1.0	70.5
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	GCTCAACCTACGGGAACTG	50.0	57.9	58.2	57.9	832-834 bp	1.0	70.0