Hotspot

ccsA-ndhD

SSC · 118503-118783 · 281 bp

Back to Hydrangea

Pi 0.0539

MarkerSeek score 71.40

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 281
variable_sites: 56
pi: 0.0539
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GCAATAACCGTTCCAAGTGC	TGCATTACCGGGAATGAGTG	50.0	50.0	58.0	58.0	1263-1297 bp	1.0	89.9
ccsA-ndhD_p2	GCAATAACCGTTCCAAGTGC	TTATCTCGACGAAATGGGCG	50.0	50.0	58.0	58.2	1344-1378 bp	1.0	88.6
ccsA-ndhD_p3	GCAATAACCGTTCCAAGTGC	GGGACTCAATGGAGCCATTT	50.0	50.0	58.0	57.9	1443-1477 bp	1.0	88.2
ccsA-ndhD_p4	GCAATAACCGTTCCAAGTGC	TTCAACATCTCCTGGCCAAC	50.0	50.0	58.0	58.1	1545-1579 bp	1.0	88.1
ccsA-ndhD_p5	GCAATAACCGTTCCAAGTGC	GCATATGGGTTGGTTCGGAT	50.0	50.0	58.0	58.0	1649-1683 bp	0.964286	86.7