Hotspot

ndhF-rpl32

SSC · 115126-116268 · 1143 bp

Back to Hydrangea

Pi 0.0402
MarkerSeek score 78.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1143
variable_sites
199
pi
0.0402
unique_haplotype_count
25
primer_available
yes
alignment_reliability
0.98
markerseek_score
78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTTGCCGTCGGAAAAAGAAG AAACGAGAGGTACATGGGGA 50.0 50.0 58.0 58.1 1519-1914 bp 0.607143 75.6
ndhF-rpl32_p2 GTTGCCGTCGGAAAAAGAAG GATATCAGAAGAGCGAGGCG 50.0 55.0 58.0 58.0 1528-1562 bp 0.392857 64.5
ndhF-rpl32_p3 TGCCGTCGGAAAAAGAAGAA GATATCAGAAGAGCGAGGCG 45.0 55.0 58.0 58.0 1526-1560 bp 0.392857 64.5
ndhF-rpl32_p4 TTGCCGTCGGAAAAAGAAGA GATATCAGAAGAGCGAGGCG 45.0 55.0 58.0 58.0 1527-1561 bp 0.392857 64.5
ndhF-rpl32_p5 CAAGAGGTCGTGTGAACCAA GATATCAGAAGAGCGAGGCG 50.0 55.0 58.1 58.0 1941-1975 bp 0.392857 63.0