Hotspot

ycf4-cemA

LSC · 62672-63548 · 877 bp

Back to Hydrangea

Pi 0.0211

MarkerSeek score 71.40

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 877
variable_sites: 103
pi: 0.0211
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	AGAAGAGGAGTGAATGCTTTCT	45.0	40.9	58.1	57.1	715-976 bp	1.0	68.0
ycf4-cemA_p2	AAAAAGCTGCTGAATTGGCC	AGAAGAGGAGTGAATGCTTTCT	45.0	40.9	57.8	57.1	729-990 bp	1.0	67.4
ycf4-cemA_p3	TTGGCCTATTTCTTGCGTGT	TGCGAGATATAGAAGAGGAGTGA	45.0	43.5	58.1	58.0	725-986 bp	1.0	67.0
ycf4-cemA_p4	AAAGCTGCTGAATTGGCCTA	AGAAGAGGAGTGAATGCTTTCT	45.0	40.9	57.8	57.1	727-988 bp	1.0	67.0
ycf4-cemA_p5	AAAAAGCTGCTGAATTGGCC	TGCGAGATATAGAAGAGGAGTGA	45.0	43.5	57.8	58.0	739-1000 bp	1.0	66.4