Hotspot

accD-psaI

LSC · 60924-61581 · 658 bp

Back to Hydrangea

Pi 0.039

MarkerSeek score 77.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 658
variable_sites: 98
pi: 0.039
unique_haplotype_count: 27
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AACGCCTACATTGCATTTGC	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	841-932 bp	1.0	91.7
accD-psaI_p2	AACGCCTACATTGCATTTGC	TACATTTCAGACATCCGCCG	45.0	50.0	58.0	58.1	1090-1181 bp	1.0	91.4
accD-psaI_p3	AACGCCTACATTGCATTTGC	TCCGCCGATCCTGATCTATT	45.0	50.0	58.0	58.1	1077-1168 bp	1.0	91.4
accD-psaI_p4	ACCCGTTTGATCGAGTATGC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	1081-1172 bp	1.0	91.3
accD-psaI_p5	TCGATTGATCCAGGCACTTG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1386-1412 bp	0.678571	77.1