Hotspot

trnF-GAA-ndhJ

LSC · 50139-50822 · 684 bp

Back to Hydrangea

Pi 0.0237

MarkerSeek score 74.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 684
variable_sites: 83
pi: 0.0237
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	737-2353 bp	1.0	91.2
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	701-2353 bp	1.0	90.9
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.9	58.1	725-2353 bp	1.0	90.2
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	ATCATCCACGCTTGAAACGT	55.0	45.0	58.0	58.2	772-2388 bp	1.0	90.0
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	ATCATCCACGCTTGAAACGT	50.0	45.0	58.1	58.2	736-2388 bp	1.0	89.9