Hotspot

trnG-GCC-trnM-CAU

LSC · 38004-38198 · 195 bp

Back to Hydrangea

Pi 0.04
MarkerSeek score 74.60
Primer available yes
Label trnG-GCC-trnM-CAU

Feature Metadata

label_name
trnG-GCC-trnM-CAU
feature_type
igs
region
LSC
length_bp
195
variable_sites
38
pi
0.04
unique_haplotype_count
25
primer_available
yes
alignment_reliability
0.97
markerseek_score
74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnM-CAU_p1 TTGCGGATATGGTCGAATGG GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 326-349 bp 1.0 90.8
trnG-GCC-trnM-CAU_p2 TTGCGGATATGGTCGAATGG CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 290-313 bp 1.0 90.7
trnG-GCC-trnM-CAU_p3 TCTTTGCCAAGGAGAAGACG GGGTAGAGCAGTTTGGTAGC 50.0 55.0 57.8 58.0 297-320 bp 1.0 90.4
trnG-GCC-trnM-CAU_p4 TCTTTGCCAAGGAGAAGACG CTTGAGGTCACGGGTTCAAA 50.0 50.0 57.8 58.0 261-284 bp 1.0 90.3
trnG-GCC-trnM-CAU_p5 TTGCGGATATGGTCGAATGG GTCACGGGTTCAAATCCTGT 50.0 50.0 58.1 58.1 284-307 bp 1.0 90.2