Hotspot

trnK-UUU-rps16

LSC · 4277-5141 · 865 bp

Back to Hydrangea

Pi 0.0371
MarkerSeek score 76.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
865
variable_sites
146
pi
0.0371
unique_haplotype_count
26
primer_available
yes
alignment_reliability
0.99
markerseek_score
76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCTTTCAGGATCAGTCGTGG TCTATCCCAATGAGCCGTCT 55.0 50.0 58.0 57.9 1186-1246 bp 0.964286 89.7
trnK-UUU-rps16_p2 CCCTTTCAGGATCAGTCGTG TGTTGCTGCCGTTTTGAAAG 55.0 45.0 58.0 58.1 1634-1693 bp 0.964286 89.3
trnK-UUU-rps16_p3 CCTTTCAGGATCAGTCGTGG TGTTGCTGCCGTTTTGAAAG 55.0 45.0 58.0 58.1 1633-1692 bp 0.964286 89.3
trnK-UUU-rps16_p4 CCCTTTCAGGATCAGTCGTG GCCGTACGAGGAGAAAACTT 55.0 50.0 58.0 57.9 1267-1305 bp 0.928571 87.7
trnK-UUU-rps16_p5 CCTTTCAGGATCAGTCGTGG GCCGTACGAGGAGAAAACTT 55.0 50.0 58.0 57.9 1266-1304 bp 0.928571 87.7