Hotspot

ycf1

SSC · 131771-137551 · 5781 bp

Back to Hydnocarpus

Pi 0.0185

MarkerSeek score 50.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5781
variable_sites: 107
pi: 0.0185
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCTTTTTCTTTCTACGTTTT	GATCTTTTGCTATCTATATCATCA	28.6	29.2	52.5	52.1	179 bp	1.0	43.6
ycf1_p2	TTGCTTTTTCTTTCTACGTTT	GATCTTTTGCTATCTATATCATCA	28.6	29.2	52.5	52.1	180 bp	1.0	43.6
ycf1_p3	TGCTTTTTCTTTCTACGTTTTT	GATCTTTTGCTATCTATATCATCA	27.3	29.2	53.4	52.1	179 bp	1.0	43.6
ycf1_p4	TTGCTTTTTCTTTCTACGTTTT	GATCTTTTGCTATCTATATCATCA	27.3	29.2	53.4	52.1	180 bp	1.0	43.6
ycf1_p5	TGCTTTTTCTTTCTACGTTTT	GATCTTTTGCTATCTATATCATCAA	28.6	28.0	52.5	52.9	179 bp	1.0	43.6