Hotspot

petD

LSC · 83704-85138 · 1435 bp

Back to Hydnocarpus

Pi 0.0153

MarkerSeek score 53.50

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1435
variable_sites: 22
pi: 0.0153
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TATCGTCCGACCGTTACTGA	CACTTCATGAAGCCTCTCGG	50.0	55.0	58.0	58.4	2712 bp	0.5	57.0
petD_p2	ACTATCGTCCGACCGTTACT	CACTTCATGAAGCCTCTCGG	50.0	55.0	58.0	58.4	2714 bp	0.5	57.0
petD_p3	TTGGCGTAACTGGCTATTCC	CACTTCATGAAGCCTCTCGG	50.0	55.0	58.0	58.4	2492 bp	0.5	56.9
petD_p4	TATCGTCCGACCGTTACTGA	AGCACTTCATGAAGCCTCTC	50.0	50.0	58.0	57.6	2714 bp	0.5	56.6
petD_p5	ACTATCGTCCGACCGTTACT	AGCACTTCATGAAGCCTCTC	50.0	50.0	58.0	57.6	2716 bp	0.5	56.6