Hotspot

ndhF

SSC · 118625-120829 · 2205 bp

Back to Hydnocarpus

Pi 0.0204

MarkerSeek score 54.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2205
variable_sites: 45
pi: 0.0204
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GCAGAATACCGTCACCCATT	ATTGCTTGACTGTCCCCTTT	50.0	45.0	58.0	57.3	2927 bp	1.0	75.5
ndhF_p2	ACAGGAACAAGAGGGATCCA	ATTGCTTGACTGTCCCCTTT	50.0	45.0	58.0	57.3	2803 bp	1.0	75.5
ndhF_p3	AAGAGGGATCCACCGAAGAA	ATTGCTTGACTGTCCCCTTT	50.0	45.0	58.0	57.3	2795 bp	1.0	75.5
ndhF_p4	GATCCGGACAAAATCGACGA	ATTGCTTGACTGTCCCCTTT	50.0	45.0	58.1	57.3	2739 bp	1.0	75.3
ndhF_p5	AGGGGGACTAAACAGGAACA	ATTGCTTGACTGTCCCCTTT	50.0	45.0	57.9	57.3	2814 bp	1.0	74.9