Hotspot

clpP

LSC · 76999-79070 · 2072 bp

Back to Hydnocarpus

Pi 0.0169

MarkerSeek score 53.70

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2072
variable_sites: 35
pi: 0.0169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CTGAACGAGTCGCACATACA	TACGGACCCCTCTTTTCAGT	50.0	50.0	58.0	58.0	2810 bp	1.0	81.2
clpP_p2	TGACCCTGTTAGTCCGTTCT	GTACCAATACGCAATGGGGT	50.0	50.0	58.0	58.0	2979 bp	1.0	81.1
clpP_p3	CTGAACGAGTCGCACATACA	GTACCAATACGCAATGGGGT	50.0	50.0	58.0	58.0	2676 bp	1.0	81.0
clpP_p4	CTGAACGAGTCGCACATACA	CCTATGTTACGGACCCCTCT	50.0	55.0	58.0	57.9	2817 bp	1.0	80.7
clpP_p5	CTAGTACATGTTCCTCGGCG	TACGGACCCCTCTTTTCAGT	55.0	50.0	57.9	58.0	2788 bp	1.0	80.4