Pi
0.0297
MarkerSeek score
60.00
Primer available
yes
Label
rps15-ycf1
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| rps15-ycf1_p1 | TGTGAAATAATTGAGATGAATGCA | TCCACGATTGAAAATTCTGT | 29.2 | 35.0 | 55.4 | 53.1 | 461 bp | 1.0 | 42.1 |
| rps15-ycf1_p2 | TGTGAAATAATTGAGATGAATGCA | ATCCACGATTGAAAATTCTGT | 29.2 | 33.3 | 55.4 | 53.8 | 462 bp | 1.0 | 42.1 |
| rps15-ycf1_p3 | TTGTGAAATAATTGAGATGAATGCA | TCCACGATTGAAAATTCTGT | 28.0 | 35.0 | 56.1 | 53.1 | 462 bp | 1.0 | 42.1 |
| rps15-ycf1_p4 | CTTGTGAAATAATTGAGATGAATGCA | TCCACGATTGAAAATTCTGT | 30.8 | 35.0 | 57.1 | 53.1 | 463 bp | 1.0 | 42.1 |
| rps15-ycf1_p5 | TGTGAAATAATTGAGATGAATGCAT | TCCACGATTGAAAATTCTGT | 28.0 | 35.0 | 55.9 | 53.1 | 461 bp | 1.0 | 42.1 |