Hotspot

rps12-clpP

LSC · 76835-76998 · 164 bp

Back to Hydnocarpus

Pi 0.0427

MarkerSeek score 62.60

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 164
variable_sites: 7
pi: 0.0427
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	TGACCCTGTTAGTCCGTTCT	CCTGGAAGCGGAAGAACTAC	50.0	55.0	58.0	58.0	765 bp	1.0	81.5
rps12-clpP_p2	CTGAACGAGTCGCACATACA	CCTGGAAGCGGAAGAACTAC	50.0	55.0	58.0	58.0	462 bp	1.0	81.5
rps12-clpP_p3	TGACCCTGTTAGTCCGTTCT	TTCCTCCTAAGGGAAGGGTG	50.0	55.0	58.0	58.0	1069 bp	1.0	81.4
rps12-clpP_p4	CTGAACGAGTCGCACATACA	TTCCTCCTAAGGGAAGGGTG	50.0	55.0	58.0	58.0	766 bp	1.0	81.3
rps12-clpP_p5	TGACCCTGTTAGTCCGTTCT	TCCTGGAAGCGGAAGAACTA	50.0	50.0	58.0	58.1	766 bp	1.0	81.1